Cytotoxicity assays are often performed as endpoint assays, but a kinetic or time-resolved assay enables more precise assessment of cytotoxicity, especially if the kinetics of the compounds are unknown or varied, or if cells display different proliferation rates.
Assessment of cytotoxicity can also be affected by the type of cell model, e.g. 2D or 3D, or the use of fluorescent dyes which, over a long time-course, can have negative effects on metabolism and proliferation and hence on responses to the exogenous factors.
In this technical note, we describe label-free analysis of cell growth in monolayer and spheroid growth in 3D using brightfield images in order to determine cytotoxic effects, and compare the responses of three different cell lines to test compounds in both 2D and 3D.
Download our technical note to learn how you can: